This is too concentrated to be assayed by the Coomassie Plus Protein Assay Kit, whose assay range in the standard microplate protocol is 100-1500g/mL. This assay was performed after a 6-h fast 1 day following the last Lac-Phe dose on day 10. Newest Tumor Compendium: v11 April 2020. Hypertrophic cardiomyopathy (HCM) is a common inherited cardiovascular disease with heterogeneous clinical presentations, governed by multiple molecular mechanisms. Pierce Protein Methods (17 h) at 4C. gg for 10 min at 4C) and the protein concentration was determined using the BCA assay (Thermo Fisher The experimental protocol was approved by the Columbia University Animal Care and Use Committee and, in accordance with National Institutes of Health (NIH) guidelines, adequate measures were taken to minimize pain and discomfort. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. The amount of retentate was estimated using either the Pierce BCA Protein Assay Kit or by absorption at 360 nm (for vitamin B12). Pierce BCA Protein Assays have a unique advantage over the Coomassie dyebased assays (Bradford)they are compatible with samples that contain up to 5% surfactants (detergents), and are affected much less by protein compositional differences, providing NPs produced from 0.02-, 0.1-, and 0.2-m NTs were filter-sterilized using 0.45-m Polyethersulfone (PES) membranes. BCA rely on the formation of a Cu 2 +protein complex in a basic environment, followed by reduction of the Cu 2 + to Cu + (Smith et al., 1985).The amount of Cu 2 + that is reduced is proportional to the amount of protein present in solution. 23227). The cell lysates were clarified by centrifugation at 14,000 x g for 10 minutes, the supernatant was collected and the protein concentration (g/million cells) was determined using the Pierce BCA Protein Assay (Cat. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. Large-size nano/microparticles (0.5 to 2 m) were prepared in aseptic conditions and were used without additional filtration. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. The Pierce 660-nm Protein Assay can be performed in either a test tube or microplate format. The concentration of protein were measured using the Pierce BCA assay (Thermo-Scientific, IL, USA). The Pierce Rapid Gold BCA Protein Assay Kit is a fast, two-component, high-precision, detergent-compatible assay optimized to determine total protein concentration. Samples were prepared at a starting concentration of 0.5 to 1mg/mL in either PBS (pH 7) or 0.2M carbonate bicarbonate buffer (pH 9.4). The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. While this isotopic assay method is rarely performed in laboratories today, the acronym for this lysis buffer formulation has endured in common use. BCA is similar to the Lowry or Bradford protein assays and was first made commercially available by Pierce, which is now owned by Thermo Fisher Scientific.In the BCA assay, a protein's peptide bonds quantitatively reduce Cu 2+ to Catalogue Mass Spectrometry Sample Preparation. Slide-A-Lyzers cassette membranes are available in these pore sizes: 2K, 3.5K, 7K, 10K and 20K. The BCA Protein Assay is a popular method for colorimetric detection and quantitation of total protein. The kit enables you to measure protein concentration in samples that contain the disulfide reducing agents. So I did the BCA assay and came up with these concentrations/ul: Sample 1 = 2.9 ug/ul, Sample 2 = 2.5 ug/ul, Sample 3 = 3.5 ug/ul, Sample 4 = 2.7 ug/ul. (BCA) method . Total protein concentration was determined using micro-BCA protein assay (Pierce). approximately 5mg/mL. This assay was performed after a 6-h fast 1 day following the last Lac-Phe dose on day 10. BSA is such a protein, and the Pierce Albumin Standards are Pierce Quantitative Colorimetric Peptide Assay provides modified BCA reagents for the reduction of Cu+2 to Cu+1, a proprietary chelator optimized for the quantitation of peptide mixtures, and peptide digest reference standard for use in the generation of control linear standard curves. The oxygen consumption rate (OCR) of whole cells was determined by using a Seahorse XFp Extracellular Flux Analyzer (Seahorse Bioscience). The Pierce Rapid Gold BCA Protein Assay Kit is a fast, two-component, high-precision, detergent-compatible assay optimized to determine total protein concentration. How the Pierce 660-nm Assay detects protein The Pierce 660-nm Protein Assay is based on the binding of a proprietary dye-metal complex to protein in acidic conditions that causes a shift in the dye's absorption maximum, which is measured at 660 nm. Cells were trypsinized and plated on a miniplate 24 hours prior to the Seahorse assay. The Micro BCA Protein Assay Kit is a specialized version of the popular Pierce BCA Protein Assay for determining the protein concentration of dilute samples. In our online shop we offer the most diverse portfolio of bioresources for researchers from academia & industry. RIPA cell lysis reagent is highly effective for protein extraction from a variety of cell types because For samples in which protein concentration is expected to be low (<20 g/mL), it may be necessary to use an assays alternate microplate protocol or use a specialized assay, such as the Pierce Micro BCA Protein Assay, which is specifically designed for dilute samples. 23215 Compat-AbleTM Protein Assay Preparation Reagent Set, sufficient reagents to pre-treat 500 samples to remove interfering substances before total protein quantitation 90036 Protein Precipitation Plates, 2 plates, for removing protein from samples before MS analysis. The concentration of proteins was measured using a BCA Protein Assay Kit (23227, Pierce) and finally the protein concentration of each sample was adjusted to the same level. Primary cortical neurons were preincubated with Irisin (50 ng/mL) for 1 h and -syn PFF was administered the neurons were further incubated with Irisin for 1 or 4 d. The Pierce 660-nm Protein Assay is a ready-to-use, detergent- and reducing agent-compatible assay reagent to quickly measure total protein concentration compared to a protein standard. Western blot assay Saliva samples were pelleted by centrifugation at 14,000 g for 15 min at 4 C. Thermo Scientific Pierce BSA Protein Assay Standards are high-quality reference samples for generating accurate standard curves and calibration controls in total protein assays.Features of BSA Protein Assay Standards: Protein assay quantitation standard (BCA Protein Assay, Coomassie-Bradford Assay, etc.) Detergent Compatible Bradford Assay. Mixing together the three Micro BCA reagents results in a working solution that is sufficiently concentrated to measure protein when mixed with an equal volume of sample. Bicinchoninic acid (BCA) assay or Smith assay is a copper-based colorimetric assay for total protein quantification. In such cases, the best standard is one that will produce a normal (i.e., average) color response curve with the selected protein assay method and is readily available to any researcher. of WT and CNDP2-KO mice following a combined obesogenic diet and treadmill running protocol. The values were normalized to protein concentrations measured from a BCA protein assay kit (Pierce) and the data were expressed in ng/mg protein. The BCA Protein Assay is a popular method for colorimetric detection and quantitation of total protein. The Tumor Compendium v11 Public PolyA is now available for download and visualization.This compendium includes RNA expression data from over 12,000 samples, including 406 newly added samples from the Therapeutically Applicable Research To Generate Effective Treatments (TARGET) program. Retention was measured using either the Thermo Scientific Pierce BCA Protein Assay (Part No. This BCA Protein Assay Kit is a reducing agent-compatible version of our popular Pierce BCA Protein Assay. For 2DG experiments, the OCR was normalized to the final cell number determined by manual cell counting. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. However, one could dilute it 5-fold in buffer (i.e., 1 part sample plus 4 parts buffer) and then use that diluted sample as thetest sample in the protein assay. No. of WT and CNDP2-KO mice following a combined obesogenic diet and treadmill running protocol. Pierce BCA Protein Assays have a unique advantage over the Coomassie dyebased assays (Bradford)they are compatible with samples that contain up to 5% surfactants (detergents), and are affected much less by protein compositional differences, providing For digestion, 20 g of each exosome protein isolate was precipitated by methanol/chloroform method. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. Pierce Detergent Compatible Bradford Assay Kit is a quick and ready-to-use modification of the well-known Bradford Coomassie dye-binding, colorimetric method for total protein quantitation. 23225) or absorption at 360nm (for vitamin B12). The bicinchoninic acid assay (BCA) is based on a simple colorimetric measurement and is the most common protein quantification assay.